comparison of lipoamide dehydrogenase activity in hl-60 leukemia cells and normal lymphocyte

to determine the importance of q 10 h 2 as an antioxidant in cancer, we measured the activity of lipoamide dehydrogenase (q 10 h 2 recycling enzyme) in hl-60 and normal lymphocyte. the cultured cells of hl-60 and human normal lymphocytes were assayed in cell lysate of given number of both hl-60 and normal lymphocyte. the activity of lipoamide dehydrogenase and the protein concentration were determined by spectrometric methods. the activity of lad was found to be 0.216µmol/min/µg protein in hl-60 cells and 0.0415µmol/min/µg protein in normal lymphocytes. although the average protein concentration ratio in hl-60 cells to normal lymphocyte was found to be %124, the average ratio of enzyme activity in hl-60 to normal lymphocyte was %526. these results are indicative of independence of enzyme increase in hl-60 cells of increase of protein synthesis.